Optimization of soluble organic selenium accumulation during fermentation of Flammulina velutipes mycelia.

Selenium accumulation in mycelia of Flammulina velutipes during fermentation determined by RP-HPLC.

A method to estimate the content of selenium in organics was introduced based on reversed phase-high performance liquid chromatography (RP-HPLC).The maximum absorption peak of piazselenol was at 330 nm and the optimized temperature and pH value were 40 degrees C and 2.8, respectively. The minimum detection concentration of selenium(IV) was 0.06 microg/mL and the measurable range was 0.12-12.0 m...

Selenium uptake, tolerance and reduction in Flammulina velutipes supplied with selenite

Recently, selenium (Se) enriched mushrooms have been exploited as dietary Se supplements, but our knowledge of the metabolic process during the Se enrichment process is far from complete. In this study, the uptake, tolerance and reduction of selenite in a widely cultivated mushroom, Flammulina velutipes, was investigated. The results showed that pH variation (from 5.5-7.5), metabolic inhibitor ...

Development of a gene transfer system for the mycelia of Flammulina velutipes Fv-1 strain.

To develop a gene transformation method for Flammulina velutipes, we constructed a vector with hph gene under control of the trp1 gene promoter. The vector was integrated into protoplast derived from mycelia by the calcium-polyethylene glycol method, as it has not been reported for F. velutipes. Transformation efficiency was much improved when transformation was performed by the restriction enz...

Selenium uptake, toxicity and reduction in Flammulina velutipes supplied with selenite

Solid-state fermentation of sugarcane bagasse with Flammulina velutipes and Trametes versicolor.

The mushroom Flammulina velutipes and the white-rot fungus Trametes versicolor were cultivated separately on sugarcane bagasse for 40 days. Trametes versicolor produced laccase and manganese-peroxidase activities, showing a simultaneous degradation of lignin and holocellulose. However, only phenoloxidase activity was found with Flammulina velutipes. A preferential degradation of lignin was dete...